α-倒捻子素通过抑制TLR4/NF-κB信号通路缓解LPS诱导IEC-6细胞的炎症

doi:10.11843/j.issn.0366-6964.2019.02.022

摘要/Abstract

摘要：

旨在证明α-倒捻子素可以缓解脂多糖（LPS）诱导的IEC-6细胞的炎症并揭示其机制。笔者在体外培养的IEC-6细胞中构建LPS诱导的炎症模型，并通过流式细胞术、酶联免疫吸附测定（ELISA）、定量PCR（Q-PCR）、蛋白印迹（Western blot）的方法检测α-倒捻子素对LPS诱导的炎症是否有缓解作用。结果表明，5 μmol·L^-1的α-倒捻子素预处理可以显著降低LPS诱导的前列腺素E2（PGE2）、白细胞介素-1β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）在IEC-6细胞中的分泌，以及环氧合酶2（COX-2）、IL-6、TNF-α、IL-1β mRNA的表达（P<0.05）。通过对炎症相关信号通路NF-κB的探究可见，α-倒捻子素能显著抑制Toll样受体4（TLR4）mRNA和NF-κB相关蛋白磷酸化IκBα（pIκBα）和磷酸化p65（pp65）的激活（P<0.05）。综上所述，α-倒捻子素可以通过TLR4/NF-κB信号通路来抑制LPS诱导的IEC-6细胞的炎症，并且可能是治疗炎症疾病的潜在选择。

Abstract:

The aim of this study was to prove that α-mangostin can alleviate the lipopolysaccharide (LPS)-induced inflammation of IEC-6 cells and reveal its mechanism. In this study, we constructed an LPS-induced inflammation model in IEC-6 cells in vitro, and examined whether LPS-induced inflammation was reduced by α-mangostin by means of the methods of rheumatology, ELISA, Q-PCR, and Western blot. The results showed that pretreatment with 5 μmol·L^-1 α-mangostin significantly reduced the secretion of prostaglandin (PG) E2, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and the expression of COX-2, IL-6, TNF-α, IL-1β mRNA induced by LPS in IEC-6 cells (P<0.05). By investigating the inflammatory-related signaling pathway, NF-κB, we found that α-mangostin significantly inhibited the activation of TLR4 mRNA and NF-κB-related protein phosphorylated IκBα (pIκBα) and phosphorylated p65 (pp65) (P<0.05). In summary, α-mangostin can inhibit LPS-induced inflammation of IEC-6 cells through the TLR4/NF-κB signaling pathway and may be a potential treatment for inflammatory diseases.

中图分类号:

S853.7

邹闻书, 尹朋, 金娜, 高倩, 刘凤华. α-倒捻子素通过抑制TLR4/NF-κB信号通路缓解LPS诱导IEC-6细胞的炎症[J]. 畜牧兽医学报, 2019, 50(2): 431-438.

ZOU Wenshu, YIN Peng, JIN Na, GAO Qian, LIU Fenghua. α-Mangostin Alleviates LPS-induced Inflammation by Inhibiting TLR4/NF-κB Signaling[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 431-438.

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https://www.xmsyxb.com/CN/Y2019/V50/I2/431

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